Journal: ACS Applied Materials & Interfaces

Article Title: Long-Term Controlled Growth Factor Release Using Layer-by-Layer Assembly for the Development of In Vivo Tissue-Engineered Blood Vessels

doi: 10.1021/acsami.2c05988

Figure Lengend Snippet: General overview of the cellular composition of the tissue capsule formed around implanted rods. (A) vWF staining for vascularization, (B) Ki67 for proliferating cells, (C) α-SMA for myofibroblast, (D) desmin for contractile smooth muscle cells, and (E) vimentin for fibroblast. The tissue capsules are mainly composed of fibroblasts and myofibroblasts. There are barely contractile smooth muscle cells present. All tissue capsules are well vascularized. Scale bar represents 50 μm.

Article Snippet: Adult rat dermal fibroblasts (aRDF, #R2320, ScienCell Research Laboratories) were cultured with a basic culture medium comprising DMEM (Gibco), fetal bovine serum (10%, Lonza), l -glutamine (2 mM, Gibco), and penicillin (100 U/mL) and streptomycin (100 mg/mL, Gibco). aRDF were expanded at an initial seeding density of 5000 cells/cm 2 in a culture medium and refreshed every 2–3 days.

Techniques: Staining, Capsules

Journal: ACS Applied Materials & Interfaces

Article Title: Long-Term Controlled Growth Factor Release Using Layer-by-Layer Assembly for the Development of In Vivo Tissue-Engineered Blood Vessels

doi: 10.1021/acsami.2c05988

Figure Lengend Snippet: (A) The number of cells (per 1000 μm 2 ) positively stained for α-smooth muscle actin is depicted, a differentiation marker for myofibroblasts. (B) The number of cells (per 1000 μm 2 ) stained positively with the desmin antibody is depicted, which stains the intermediate filament protein expressed in contractile smooth muscle cells. (C) The number of cells (per 1000 μm 2 ) positively stained for vimentin is depicted, which is an intermediate filament expressed in fibroblasts. The expressions of myofibroblasts, contractile smooth muscle cells, and fibroblasts were observed in the following conditions: control (no release), burst release (TGF-β1 and PDGF-BB), single layer-by-layer release (TGF-β1, PDGF-BB, and IGF-1), and dual layer-by-layer release (TGF-β1/IGF-1 and PDGF-BB/TGF-β1). Data was analyzed using ordinary one-way ANOVA followed by a post hoc analysis using Tukey’s test, and * indicates significance of P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

Article Snippet: Adult rat dermal fibroblasts (aRDF, #R2320, ScienCell Research Laboratories) were cultured with a basic culture medium comprising DMEM (Gibco), fetal bovine serum (10%, Lonza), l -glutamine (2 mM, Gibco), and penicillin (100 U/mL) and streptomycin (100 mg/mL, Gibco). aRDF were expanded at an initial seeding density of 5000 cells/cm 2 in a culture medium and refreshed every 2–3 days.

Techniques: Staining, Marker, Control

Journal: Nature Communications

Article Title: Pancreatic tumor eradication via selective Pin1 inhibition in cancer-associated fibroblasts and T lymphocytes engagement

doi: 10.1038/s41467-022-31928-7

Figure Lengend Snippet: a Establishment and validation of CAFs via high expressions of both FAP-α and α-SMA. b Cellular uptake of Cy5-labeled formulations on Pan02 cells, NIH-3T3 cells, and CAFs, measured by flow cytometry ( n = 3 biologically independent experiments; error bars, mean ± SD). c Cellular uptake of Cy5-labeled antiCAFs-DMS on cells, imaged by fluorescent microscopy. Scale bars are 20 μm. Three independent repetitions got similar results. d Cellular uptake of Cy5-labeled antiCAFs-DMS on cells, quantified by flow cytometry ( n = 6 biologically independent samples; error bars, mean ± SD). Cytotoxicity on Pan02 cells ( e ) and CAFs ( f ) ( n = 3 biologically independent experiments; error bars, mean ± SD). g Western blotting detection of Pin1 and relative proteins from Pan02 cells after treating with PBS, AG17724 (0.5 μM), or antiCAFs-DMS (corresponds to 0.5 μM of AG17724) for 24 hours. h Quantitative analysis of the western blotting results from Pan02 cells (Three independent repetitions got similar results.). i Western blotting detection of Pin1 and relative proteins from CAFs treated with PBS, AG17724, or antiCAFs-DMS after treating with PBS, AG17724 (0.5 μM) or antiCAFs-DMS (corresponds to 0.5 μM of AG17724) for 24 hours. j Quantitative analysis of the western blotting results from CAFs (Three independent repetitions got similar results.). One-way analysis of variance followed by Turkey posttests. Source data are provided as a Source Data file.

Article Snippet: Anti-FAP-α antibody (MAB9727-100, 0.25 μg/mL as working concentration.) and anti-phospho-CDC2/CDK1 (Y15) antibody (AF888-SP, 0.2 μg/mL as working concentration.) were purchased from R&D SYSTEMS.

Techniques: Biomarker Discovery, Labeling, Flow Cytometry, Microscopy, Western Blot